16S rDNA Based Genomic Analysis of a Bacterium Isolated from Earthworm (Lennogaster pusillus, Stephenson) Midden

The present study dealt with the identification of a bacterium having dominance in earthworm (Lennogaster pusillus) midden by 16S rDNA based genomic analysis. The bacterium identified was Aeromonas punctata strain JM10 (Genbank Accession No: GU205197.1). Isolated DNA of the bacterium showed amplicon band of 1500 bp, when resolved on agarose gel. Amplification of the 16S rDNA gene from the Aeromonas sp. was done with forward primer having 814 bp and reverse primer having 913 bp, which further provide consensus sequence of 1418 bp. The query sequence of 1418 bp matched the alignments scores of >= 200 in the distribution of 100 BLAST hits. About 10 types of homolog strains of Aeromonas punctata were found as its closest relatives on the basis of sequence producing significant alignments.